Kelly Lab - Washington University School of Medicine


KELLY LAB PROTOCOL BOOK
Adenovirus ATPase Muscle Histology ChIP Buffers ChIP Protocol Citrate Synthase Activity Cloning Heterologous Constructs DNA Extraction from Cells or Tissue for mtDNA Quantification Extraction of Genomic DNA from tails Fatty Acid Oxidation in Fibroblasts GeneChip Protocol Glucose Oxidation Glucose Tolerance Test GLUT 1-4 Westerns Glycogen Assay Glycogen Data, Blank Template Immunohistochemistry for PPARalpha Immunoprecipitation Insulin Tolerance Test IRS Immunoprecipitation Lactate Measurements from Mouse Tail Ligation of cDNA into Vector (Subcloning) Mitochondrial Isolation (Heart) Mitochondrial Isolation from Skeletal Muscle Mitochondrial Respiration - Skinned Fiber Murine Fibroblasts (from tail clip) Perfusion Fixation of Whole Mouse Pierce Protein Determination Preparation of Nuclear Proteins from Tissue Preparation of Membrane Proteins from Human Tissue Samples QIAGEN Plasmid Maxi Prep Rat Neonatal Cardiocyte Prep Reporter Assay using Dual-Glo Respiration Buffers for Skinned Fibers RNA Preparation and Northern Blot RNA Preparation from Cells in Culture SDH Muscle Histology Sequencing Southern (DNA) STORM Phosphorimager TAQMAN Transgenic Mice Generation Transient Transfections Tissue Prep for SDH and ATPase Stain Western Protocol Whole Cell Extracts (Cells) Whole Cell Extracts (Tissue) Whole Cell Protein Prep
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Adenovirus

ATPase Muscle Histology

ChIP Buffers

ChIP Protocol

Citrate Synthase Activity

Cloning Heterologous Constructs

DNA Extraction from Cells or Tissue for mtDNA Quantification

Extraction of Genomic DNA from tails

Fatty Acid Oxidation in Fibroblasts

GeneChip Protocol

Glucose Oxidation

Glucose Tolerance Test

GLUT 1-4 Westerns

Glycogen Assay

Glycogen Data, Blank Template

Immunohistochemistry for PPARalpha

Immunoprecipitation

Insulin Tolerance Test

IRS Immunoprecipitation

Lactate Measurements from Mouse Tail

Ligation of cDNA into Vector (Subcloning)

Mitochondrial Isolation (Heart)

Mitochondrial Isolation from Skeletal Muscle

Mitochondrial Respiration - Skinned Fiber

Murine Fibroblasts (from tail clip)

Perfusion Fixation of Whole Mouse

Pierce Protein Determination

Preparation of Nuclear Proteins from Tissue

Preparation of Membrane Proteins from Human Tissue Samples

QIAGEN Plasmid Maxi Prep

Rat Neonatal Cardiocyte Prep

Reporter Assay using Dual-Glo

Respiration Buffers for Skinned Fibers

RNA Preparation and Northern Blot

RNA Preparation from Cells in Culture

SDH Muscle Histology

Sequencing

Southern (DNA)

STORM Phosphorimager

TAQMAN

Transgenic Mice Generation

Transient Transfections

Tissue Prep for SDH and ATPase Stain

Western Protocol

Whole Cell Extracts (Cells)

Whole Cell Extracts (Tissue)

Whole Cell Protein Prep